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KMID : 0355519880120020127
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Korean Journal of Oral Biology
1988 Volume.12 No. 2 p.127 ~ p.134
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EFFECTS OF BARIUM, STRONTIUM AND COBALT ON ACINAR CELL MEMBRANE POTENTIAL AND RESISTANCE IN THE RAT SUBMAXILLARY GLAND
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Abstract
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This experiment was performed to elucidate the role of calcium and the effect of barium, strontium and cobalt on the membrane potential and input resistance of salivary acinar cells in submaxillary gland of rats.
Segments of submaxillary gland were isolated and superfused with physiological salt solution which contain Tris aminomethan buffer. pH was adjusted to 7.4 at 37C with hydrochloric acid. Membrane potential and input resistance were measured with glass microelectrode filled with 3M KC1, having a tip resistance of 1030MQ.
Resting membrane potential(RMP) and input resistance(IR) of submaxillary gland cells were -31.1mV and 12.8MQ, respectively and acetylcholine (Ach) evoked hyperpolarization by 1:3.0mV in Tris buffered solution. In calcium free solution which contain EGTA(10-4M), RMP and IR were both decreased and Ach evoked no change in membrane potential(MP) and IR. In Tris buffered solution containing barium, RMP was incresed and IR was enhanced, Ach evoked depolarization by 9.0rnV and reduction in input resistance from 25.8M Q to 20.8M Q . Strontium also increased RMP and enhanced IR and Ach evoked hyperpolarization by 6.3mV and reduction in IR from 18.7M Q to 12.7M Q. In Tris buffered solution containing cobalt, RMP and IR were also decreased but Ach evoked hyperpolarization by 6.8mV.
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KEYWORD
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